donkey α mouse cy3 antibody  (Jackson Immuno)

Journal: bioRxiv

Article Title: ATF4 and EcR interact to mediate both transcriptional activation and repression in the Drosophila fat body

doi: 10.64898/2026.03.10.710866

Figure Lengend Snippet: A) RT-qPCR analysis of indicated transcripts in isolated fat bodies from female animals expressing UAS transgenes encoding control ( LacZ RNAi ), ATF4 RNAi or EcR RNAi . UAS -transgene expression is driven by Dcg-GAL4 . Data represent average of four biological replicates and error bars represent standard error of mean. B) Representative confocal images showing 4E-BP intron -DsRed reporter expression (magenta) in female fat bodies also expressing indicated RNAi lines driven by Dcg-GAL4 . Nuclei are counterstained with DAPI (cyan). C) Quantification of nuclear DsRed in individual adipocytes from B . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. D) Relative Change in FRET after Acceptor (ATF4-GFP labeled with Cy5) photobleach upon excitation of the donor (EcR labeled with Cy3) in the nuclei of adipocytes from control ( w 1118 ) or ATF4-GFP expressing ( crc GFSTF /+ ) female animals. Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( w 1118 ) sample. Scale bar=50 μm. ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

Article Snippet: Samples were incubated for 1 hour in the dark in donkey α-mouse Cy3 antibody (1:1000, Jackson ImmunoResearch #715-165-150) and donkey α-chicken Cy5 antibody (1:1000, Jackson ImmunoResearch #703-175-155) diluted in PBS.

Techniques: Quantitative RT-PCR, Isolation, Expressing, Control, Labeling

Journal: bioRxiv

Article Title: ATF4 and EcR interact to mediate both transcriptional activation and repression in the Drosophila fat body

doi: 10.64898/2026.03.10.710866

Figure Lengend Snippet: A) Representative confocal images showing 4E-BP intron -GFP reporter expression (magenta) in fat bodies also expressing LacZ RNAi , either ATF4 RNAi or usp RNAi , and both usp RNAi and ATF4 RNAi driven by Dcg-GAL4. Equal number of UAS-transgene cassettes were used by combining RNAi lines with either LacZ or LacZ RNAi . B) Quantification of nuclear GFP in individual adipocytes from A . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. C) Relative Change in FRET after Acceptor (Usp-GFP labeled with Cy5) photobleach upon excitation of the donor (EcR labeled with Cy3) in the nuclei of wandering third instar larval fat bodies where Dcg-GAL4 drives expression of control ( LacZ RNAi ) or ATF4 RNAi . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. Scale bar=50 μm. ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

Article Snippet: Samples were incubated for 1 hour in the dark in donkey α-mouse Cy3 antibody (1:1000, Jackson ImmunoResearch #715-165-150) and donkey α-chicken Cy5 antibody (1:1000, Jackson ImmunoResearch #703-175-155) diluted in PBS.

Techniques: Expressing, Control, Labeling

Journal: Cell Transplantation

Article Title: Transplantation Efficacy of Human Ciliary Epithelium Cells from Fetal Eye and Lin-ve Stem Cells from Umbilical Cord Blood in the Murine Retinal Degeneration Model of Laser Injury

doi: 10.1177/0963689720946031

Figure Lengend Snippet: List of Antibodies Used in Immunohistochemical Experiments Real-Time PCR.

Article Snippet: 3. , Cy3 conjugated Donkey α Rabbit , Jackson Immunoresearch, USA , 1:200.

Techniques: Immunohistochemical staining

Journal: Cell Transplantation

Article Title: Transplantation Efficacy of Human Ciliary Epithelium Cells from Fetal Eye and Lin-ve Stem Cells from Umbilical Cord Blood in the Murine Retinal Degeneration Model of Laser Injury

doi: 10.1177/0963689720946031

Figure Lengend Snippet: Differentiated CE cells transplantation enhances rod cells expression. (A) Immunohistochemistry of rhodopsin showing Cy3 (red fluorescence marked by arrows) expression bound to primary antibody at 20× visualized under confocal microscope in normal control, laser injured retina mice, laser injured transplanted with Lin-ve SC mice, and laser injured transplanted with CE cells mice. (B) Quantitative protein expression of rhodopsin at 1-week time point measured by corrected total cell fluorescence of immunohistochemistry images using Image J software. (C) mRNA expression of rhodopsin in all four groups was analyzed using real-time PCR at 1 week and 3 months after transplantation. Statistical analysis was performed using one-way ANOVA test for immunohistochemistry and real-time PCR results. This was followed by post-hoc analysis using LSD, Scheffe, and Dunnett’s test. * P ≤ 0.05 and ** P ≤ 0.01 were regarded as statistically significant. ANOVA: analysis of variance; CE: ciliary epithelium; Lin-ve SC: lineage negative stem cells; PCR: polymerase chain reaction.

Article Snippet: 3. , Cy3 conjugated Donkey α Rabbit , Jackson Immunoresearch, USA , 1:200.

Techniques: Transplantation Assay, Expressing, Immunohistochemistry, Fluorescence, Microscopy, Control, Software, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

Journal: Cell Transplantation

Article Title: Transplantation Efficacy of Human Ciliary Epithelium Cells from Fetal Eye and Lin-ve Stem Cells from Umbilical Cord Blood in the Murine Retinal Degeneration Model of Laser Injury

doi: 10.1177/0963689720946031

Figure Lengend Snippet: Differentiated CE cell transplantation enhances expression of retinal ganglion cells. (A) Immunohistochemistry of Thy1 showing Cy3 (red fluorescence marked by arrows) expression bound to primary antibody at 20× visualized under confocal microscope in normal control mice, laser injured retina mice, laser injured transplanted with Lin-ve SC mice, and laser injured transplanted with CE cells mice. (B) Quantitative protein expression of Thy1 at 1 week after transplantation was measured by corrected total cell fluorescence of immunohistochemistry images using Image J software (C). mRNA expression of Thy1 in all four groups was analyzed using real-time PCR at 1 week as well as 3 months after transplantation. Statistical analysis was performed using one-way ANOVA test for immunohistochemistry and real-time PCR results. This was followed by post hoc analysis using LSD, Scheffe, and Dunnett’s test. * P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001 were regarded as statistically significant. ANOVA: analysis of variance; CE: ciliary epithelium; Lin-ve SC: lineage negative stem cells; PCR: polymerase chain reaction.

Article Snippet: 3. , Cy3 conjugated Donkey α Rabbit , Jackson Immunoresearch, USA , 1:200.

Techniques: Transplantation Assay, Expressing, Immunohistochemistry, Fluorescence, Microscopy, Control, Software, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

Journal: Cell Transplantation

Article Title: Transplantation Efficacy of Human Ciliary Epithelium Cells from Fetal Eye and Lin-ve Stem Cells from Umbilical Cord Blood in the Murine Retinal Degeneration Model of Laser Injury

doi: 10.1177/0963689720946031

Figure Lengend Snippet: Neurotrophic factor modulation by the Lin-ve SC transplantation. (A) Immunohistochemistry of BDNF showing Cy3 (red fluorescence marked by arrows) expression bound to primary antibody at 20× visualized under confocal microscope in normal control, laser injured mice transplanted with Lin-ve SC, and laser injured mice transplanted with CE cells. (B) Quantitative protein expression of BDNF at 1-week time point measured by corrected total cell fluorescence of immunohistochemistry images using Image J software. (C) mRNA expression of BDNF in all four groups was analyzed using real-time PCR at 1-week as well as 3-month time point. Statistical analysis was performed using one-way ANOVA test for immunohistochemistry and real-time PCR results. This was followed by post hoc analysis using LSD, Scheffe, and Dunnett’s test. * P ≤ 0.05 was regarded as statistically significant. ANOVA: analysis of variance; BDNF: brain-derived neurotrophic factor; CE: ciliary epithelium; Lin-ve SC: lineage negative stem cells; PCR: polymerase chain reaction.

Article Snippet: 3. , Cy3 conjugated Donkey α Rabbit , Jackson Immunoresearch, USA , 1:200.

Techniques: Transplantation Assay, Immunohistochemistry, Fluorescence, Expressing, Microscopy, Control, Software, Real-time Polymerase Chain Reaction, Derivative Assay, Polymerase Chain Reaction

Journal: Cell Transplantation

Article Title: Transplantation Efficacy of Human Ciliary Epithelium Cells from Fetal Eye and Lin-ve Stem Cells from Umbilical Cord Blood in the Murine Retinal Degeneration Model of Laser Injury

doi: 10.1177/0963689720946031

Figure Lengend Snippet: CE cell transplantation upregulated ciliary neurotrophic factor in the laser injured retina. (A) Immunohistochemistry of CNTF showing Cy3 (red fluorescence marked by arrows) expression bound to primary antibody at 20× visualized under confocal microscope in normal control, laser injured mice, laser injured mice transplanted with Lin-ve SC, and laser injured mice transplanted with CE cells. (B) Quantitative protein expression of CNTF at 1-week time after transplantation measured by corrected total cell fluorescence of immunohistochemistry images using Image J software. (C) mRNA expression of CNTF in all four groups was analyzed using real-time PCR at 1 week as well as 3 months after transplantation. Statistical analysis was performed using one-way ANOVA for immunohistochemistry and real-time PCR results. This was followed by post hoc analysis using LSD, Scheffe, and Dunnett’s test. * P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001 were regarded as statistically significant. ANOVA: analysis of variance; CE: ciliary epithelium; CNTF: ciliary neurotrophic factor; Lin-ve SC: lineage negative stem cells; PCR: polymerase chain reaction.

Article Snippet: 3. , Cy3 conjugated Donkey α Rabbit , Jackson Immunoresearch, USA , 1:200.

Techniques: Transplantation Assay, Immunohistochemistry, Fluorescence, Expressing, Microscopy, Control, Software, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction